Precise genome editing with base editors
نویسندگان
چکیده
Abstract Single-nucleotide variants account for about half of known pathogenic genetic in human. Genome editing strategies by reversing point mutations with minimum side effects have great therapeutic potential and are now being actively pursued. The emerge precise efficient genome such as base prime provide powerful tools nucleotide conversion without inducing double-stranded DNA breaks (DSBs), which shown curing disorders. A diverse toolkit editors has been developed to improve the efficiency accuracy different context application. Here, we summarized evolving (BEs), their limitations future perspective editing-based strategies.
منابع مشابه
Precise Editing of a Target Base in the Rice Genome Using a Modified CRISPR/Cas9 System.
CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated Cas9) has been widely used in genome editing in a variety of organisms, including rice (Cong et al., 2013; Feng et al., 2013). The majority of the editing events reported in plants typically result in an abundance of random insertions and deletions (Indels) at the target locus through the error-prone non-ho...
متن کاملBiasing genome-editing events toward precise length deletions with an RNA-guided TevCas9 dual nuclease.
The CRISPR/Cas9 nuclease is commonly used to make gene knockouts. The blunt DNA ends generated by cleavage can be efficiently ligated by the classical nonhomologous end-joining repair pathway (c-NHEJ), regenerating the target site. This repair creates a cycle of cleavage, ligation, and target site regeneration that persists until sufficient modification of the DNA break by alternative NHEJ prev...
متن کاملGenome Editing With Targeted Deaminases
Luhan Yang, Adrian W. Briggs*, Wei Leong Chew*, Prashant Mali, Marc Guell, John Aach, Daniel Bryan Goodman, David Cox, Yinan Kan, Emal Lesha Venkataramanan Soundararajan, Feng Zhang, George Church 1 Department of Genetics, Harvard Medical School, Boston, MA 2 Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, MA 3 Harvard-MIT Division of Health Science and Technology...
متن کاملGenerating Domain-Specific Model Editors with Complex Editing Commands
In software system development, domain-specific visual notations are increasingly used and need a tool environment consisting of visual editors, simulators, model transformers, etc. Several Eclipse projects head for a meta technology to define domain-specific modeling languages. The Eclipse Modeling Framework (EMF) [5] can be used to define the underlying models of visual editors. Given an EMF ...
متن کاملSpecifying and Generating Program Editors with Novel Visual Editing Mechanisms
Language specific program editors have an unenviable reputation; they are generally regarded as not having fulfilled the potential that they appear to offer. On the other hand, editing mechanisms such as direct manipulation have been very effective in other editing contexts, such as in drawing editors. It may be that these kinds of editing mechanisms would be effective in program editors, but i...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Medical review
سال: 2023
ISSN: ['2749-9642', '2097-0773']
DOI: https://doi.org/10.1515/mr-2022-0044